Functional Characterization of TRP Channels

Creative Bioarray is committed to developing a variety of strategies to provide clients with rich functional information about TRP channels. Our high-quality and detailed TRP channel function characterization service will accelerate your research on the development of drugs for the treatment of various diseases targeting TRP proteins.

Introduction

TRP channels play important functions in chemical and physical information perception and central information processing. As the pioneer of the sensory system, TRP channels are quite conservative in evolution and widely exist in lower microorganisms to higher vertebrates, including yeast, algae, worms, insects, and mammals. The different physiological functions and regulatory mechanisms of TRPs affect their relationship with diseases and targeting one or more TRPs can alleviate corresponding symptoms. Therefore, most TRPs have received extensive attention as targets for drug development and discovery.

Early drugs targeting TRP channels were focused on pain relievers. With a better understanding of TRP channels, the scope of drug discovery has expanded to more diseases, such as neurological and psychiatric diseases, diabetes, respiratory diseases, and cancer. Understanding the role of TRP channels in the occurrence and development of these diseases will help to accelerate the potential diagnosis and treatment strategies targeting TRP proteins.

Fig. 1 TRP channels expressed in the nervous system cells. (Duitama, 2020)

Our Services

Our services include but not limited to:

• Expression and function analysis of TRP channels in Saccharomyces cerevisiae.
  We have established mammalian TRP channels heterologous expression system in yeast to help clients complete the expression and function research of TRP channels. This method has the advantages of cost-effectiveness, fast cell growth, and ease of production of large numbers of cultures. Moreover, yeast has a conservative protein folding and assembly mechanism and can express a variety of mammalian membrane proteins in functional forms.
• Biochemical study of purified TRP channels ­—— Measurement of the interaction of purified TRP channels with other proteins and regulatory small molecules.
• Functional research and genetic screening of TRP channels ­—— Analysis of the structure-function relationships in TRP channels using the power and ease of yeast genetic screens.
• Analysis of the functions of TRP channels using gene knockout technology.
• Examination of the physiological processes that the TRP channels may be involved using agonists and inhibitors to assess the TRP channel functions.
• Generation of a structure-based alignment of the transmembrane domains.
  With the development of structural characterization techniques, we have determined the structures of various TRP channels and obtained the structural basis for their functional properties and pharmacology. Based on this information, we provide alignment of TRP channel transmembrane domain structures to help clients explore TRP channel functional mechanisms.

Applications

• Research on the function and physiopathological significance of TRP channels
• Research on structure-functional intimacies of TRP channels
• Drug discovery

Creative Bioarray is committed to providing clients with detailed functional characterization services of TRP channels, aiming at a deeper understanding of the structure-function relationship in TRP channels, providing crucial information for clarifying the physiological role of these channels, the pathogenesis of related diseases, and the role of drug compounds. If you are interested in our services, please contact us for more details.

Reference

1. Duitama, M.; et al. TRP channels role in pain associated with neurodegenerative diseases. Frontiers in Neuroscience, 2020, 14: 782.