Ion Channel Localization

Ion channels are located within the membranes of all excitable cells and many intracellular organelles and play an extremely important role within the nervous system. Although a detailed mechanistic understanding of ion channel function has been extensively reported in the literature, work on ion channel localization including the identification of specific sites of ion channels and their receptors on cells has long been neglected. From the pharmacological perspective, understanding information about the distribution of channel proteins, including their location in tissues and ultimately in individual cells, is as important as understanding the mechanisms by which the channels work.

Equipped with a team of researchers with extensive expertise at the forefront of the ion channel field and an advanced technology platform, Creative Bioarray is committed to developing a variety of efficient techniques to help our clients identify the exact cellular sites of ion channels and receptors. We offer ion channel localization services through a variety of strategies such as immunofluorescent labeling, high-affinity toxins, fluorescent or radioactive labeling, and green fluorescent protein (GFP) based visualization methods. We are well placed to provide a wide range of scientific services in ion channels and provide our clients with comprehensive and informative details on ion channel distribution in cells, tissues, and organisms.

Fig. 1 Ion channel localization and basic electrophysiological characterization of human-induced pluripotent stem cell-derived cardiomyocytes (hiPS-CMs). (Gélinas, 2017)

Our Services

• Imaging of Calcium Channels in Living Cells
  We are committed to developing new imaging techniques to help clients elucidate the localization and dynamic embedding of Ca²⁺ channels in the plasma membrane.
• Mapping of N-Type Ca²⁺ Channel Distributions
  We provide clients with N-type voltage-dependent calcium channels (N-VDCCs) mapping services using analogs of ω-CgTx.
• Imaging of Calcium Signals
  We help clients study the distribution and concentration changes of Ca²⁺ signals in cells and tissues.
• Localization of K⁺ Channels
  We have developed powerful methods for the quantitative ultrastructural analysis of K⁺ channel subcellular localization.
• Localization of GABA Receptors
  We provide clients with GABA_A receptors localization services, providing useful information for drug development projects in neurological diseases.
• Imaging of Nicotinic Receptors
  We help clients localize and quantify the expression of nAChRs during neuromuscular junction development and in the central nervous system.
• Mapping of Neurotransmitter Receptors
  We provide clients with mapping services for the distribution and function of neurotransmitter receptors.
• Localization of ATP-gated P2X Channels
  We provide clients with high-quality localization services for ATP-gated P2X channels and help them explore the ATP binding sites of P2X receptors.
• Localization of CFTR Ion Channel
  We focus on developing various experimental methods for localizing CFTR and studying its polarised trafficking.
• Imaging of Reconstituted Ion Channels
  We help clients image the 3D surface morphology of a wide range of reconstituted ion channels, pumps, and receptors.

Advantages of Us

• An experienced electrophysiologists team
• Extensive experience in the field of ion channels
• The highest level of technical support
• No unexpected expenses

Creative Bioarray has built lasting partnerships with clients over the years through teamwork. We offer a range of ion channel localization services to meet your extensive scientific needs. In addition, we customize more appropriate services and strategies according to your project needs to accelerate your research. If you are interested in our services, please contact us for more details.

Reference

1. Gélinas, R.; et al. Characterization of a human induced pluripotent stem cell-derived cardiomyocyte model for the study of variant pathogenicity: validation of a KCNJ2 mutation. Circulation: Cardiovascular Genetics, 2017, 10(5): e001755.