Chemical Synthesis of Ion Channels

Membrane proteins, especially transmembrane ion channels, are important targets for biotechnology research and drug development. Total chemical synthesis and semi-synthesis of proteins offer nearly limitless possibilities for the structure of these ion channels and have become widely used tools for altering and controlling the chemical structure of soluble proteins. The rapid development of Creative Bioarray in recent years in chemoselective ligation chemistry and polymer synthesis starting with synthetic and recombinantly produced protein fragments has laid the foundation for helping clients obtain different types of channel proteins.

Advanced ion channel synthesis technology, combined with highly sensitive functional analysis, such as single channel recording, will produce proteins with new and defined biological, biophysical, and biochemical characteristics, opening up a new way for elucidating the structure as well as functions in vitro and in vivo of more complex membrane proteins. At present, many articles on membrane protein synthesis have been reported. For example, the latest development of Fmoc-based SPPS, such as the depsipeptide technology and pseudoproline temporary backbone protection, has greatly accelerated the development of ion channel synthesis.

Fig. 1 Total chemical synthesis and semisynthesis of membrane proteins containing 1 or 2 transmembrane domains by native chemical ligation. (Olschewski, 2008)

Our Services

Based on our excellent research team and leading technology platform, Creative Bioarray provides clients with high-quality customized ion channel production services. We have established a variety of ion channel preparation strategies and optimized the strategies according to the specific requirements of clients to synthesize complex post-translationally modified channel proteins. We mainly provide clients with semi-synthetic and synthetic services of ion channels for structural analysis, assay development, drug discovery, etc.

• Semi-Synthesis of Ion Channels
  Our research team provides clients with semi-synthetic ion channels and incorporates a wide range of chemical modifications to part of the protein that is generated synthetically, such as backbone modifications. Protein trans-splicing (PTS) and tandem protein trans-splicing (tPTS) methods are our common strategies.
• Synthesis of Ion Channels
  We are committed to helping clients synthesize ion channels through a variety of strategies to simulate the structure and function (transport ions selectively and rectification) of ion channels on cell membranes in organisms, providing useful information for in-depth research on ion channel diseases and the development of specific therapeutic drugs.

Advantages

• Unparalleled expertise in the field of ion channels
• Advanced equipment and experienced technicians for membrane protein synthesis
• Flexible solutions to quickly adapt to changing needs of clients

Creative Bioarray has always pursued excellence in the field of ion channel research to provide clients with high-quality, professional, and reliable services. We provide the synthesis services of ion channels according to the specific scientific research needs of our clients, including semi-synthesis and synthesis services of ion channels. If you are interested in our services, please feel free to contact us for more information or a detailed discussion.

Reference

1. Olschewski, D.; Becker, C. F. W. Chemical synthesis and semisynthesis of membrane proteins. Molecular BioSystems, 2008, 4(7): 733-740.